个人简介

柏林，研究员，北京大学博雅青年学者；2012年博士毕业于中国科学院生物物理研究所，2012至2016年在美国布鲁克海文国家实验室从事博士后研究，2016至2020年在美国Van Andel 研究所担任高级研究科学家；主持国自然面上项目、北京市自然科学基金面上项目、海外高层次人才青年项目等；以通讯作者（含共同）在 Nature、Nat Commun、Sci Adv、Protein Cell、Cell Reports、Elife 等国际期刊发表研究论文；课题组主要围绕药物靶点与新药研发展开研究，重点关注抗真菌药物靶点发现与基于结构的抗真菌药物研发。

联系方式

北京大学基础医学院生物物理学系

E-mail: lbai@pku.edu.cn

研究方向

药物靶点机制研究和基于结构的新药研发。

学习与工作经历

2002.9-2006.7兰州大学 学士

2006.9-2012.8中国科学院生物物理研究所 博士

2012.8-2016.7 美国布鲁克海文国家实验室 博士后

2016.7-2020.8 美国Van Andel研究所 高级研究科学家

2020.9至今 北京大学基础医学院 研究员

授课课程（粗体为课程负责人）

本科生:《医学中的理工信下》《PBL》《生物医学图像人工智能分析入门》

研究生:《生物医学中的电镜方法》《医用生物物理学-膜与生物大分子》《分子药理学和药物发现及技术》《结构生物学研究技术》《文献阅读与写作》《生物大分子相互作用》《血管医学交叉学科思维培养》

科研团队

左起：王乐瑄、游子龙、倪悦然、吕瑞卿、柏林（导师）、王昭彬、季萌、孙雷、姜金艳、刘坤昭

科研成果

基金项目：

1. 国家自然科学基金面上项目，2022-2025

2. 优秀青年科学基金项目（海外），2022-2025

3. 北京市自然科学基金面上项目，2025-2027

4. 国家自然科学基金面上项目，2026-2029

代表研究论文：（通讯作者：*；第一作者：#）

1. You Z#, Ni Y, Song Y, Li H, Liu K, Wang L, Zhao C, Zhao P, Chen D, Wang L, Wang K, Xia P, Gu Y, Yun C, Bai L*. Assembly and cooperative mechanism of the hexameric fungal plasma membrane H+-ATPase. Cell Reports. 2025 Jun 24;44(6):115753.

2. Zhao C#, You Z, Bai L*. Fungal Plasma Membrane H+-ATPase: Structure, Mechanism, and Drug Discovery. J Fungi (Basel). 2024 Apr 8;10(4):273. doi: 10.3390/jof10040273.

3. Zhao C#, You Z#, Chen D#, Hang J#*, Wang Z, Wang L, Zhao P, Qiao J*, Yun C*, Bai L*. Structure of a fungal 1,3-β-glucan synthase. Sci Adv. 2023 Sep 15;9(37):eadh7820.

4. Chen D#, Wang Z#, Wang L, Zhao P, Yun C*, Bai L*. Structure, catalysis, chitin transport, and selective inhibition of chitin synthase. Nat Commun. 2023 Aug 8;14(1):4776.

5. Bai L*, Li H*. Structural insights into the membrane chaperones for multi-pass membrane protein biogenesis. Curr Opin Struct Biol. 2023 Apr;79:102563. doi: 10.1016/j.sbi.2023.102563.

6. Zhao P#, Zhao C#, Chen D, Yun C, Li H*, Bai L*. Structure and activation mechanism of the hexameric plasma membrane H+-ATPase. Nat Commun. 2021 Nov 8;12(1):6439.

7. Bai L*#, Jain BK#, You Q#, Duan HD, Takar M, Graham TR*, Li H*. Structural basis of the P4B ATPase lipid flippase activity. Nat Commun. 2021 Oct 13;12(1):5963.

8. Bai L*, Li H*. Cryo-EM structures of the endoplasmic reticulum membrane complex. FEBS J. 2021 Feb 24. doi: 10.1111/febs.15786.

9. Bai L*, Li H*. Protein N-glycosylation and O-mannosylation are catalyzed by two evolutionarily related GT-C glycosyltransferases. Curr Opin Struct Biol. 2021 Jan 11;68:66-73.

10. Bai L*#, You Q#, Jain BK#, Duan HD, Kovach A, Graham TR, Li H*. Transport mechanism of P4 ATPase phosphatidylcholine flippases. Elife. 2020 Dec 15;9:e62163.

11. Bai L*, You Q, Feng X, Kovach A, Li H*. Structure of the ER membrane complex, a transmembrane-domain insertase. Nature. 2020 Aug;584(7821):475-478.

12. Bai L*, Kovach A, You Q, Hsu HC, Zhao G, Li H*. Atomic structure of the eukaryotic lipid flippase Drs2p-Cdc50p. Nat Commun. 2019; 10:4142

13. Bai L*, Li H*. Cryo‐EM is uncovering the mechanism of eukaryotic protein N‐glycosylation. FEBS J. 2019; 286.9: 1638-1644.

14. Bai L#, Kovach A, You Q, Kenny A, Li H*. Structure of the eukaryotic protein O-mannosyltransferase Pmt1-Pmt2 complex. Nat Struct Mol Biol. 2019. 26:704-711

15. Bai L#, Wang T, Zhao G, Kovach A, Li H*. The atomic structure of a eukaryotic oligosaccharyl transferase complex. Nature. 2018; doi:10.1038/nature25755

16. Santos RLA#, Bai L#, Singh PK#, Murakami N#, Fan H, Zhan W, Zhu Y, Jiang X, Zhang K, Assker JP, Nathan CF, Li H*, Azzi J*, Lin G*. Structure of human immunoproteasome with a reversible and noncompetitive inhibitor that selectively inhibits activated lymphocytes. Nat Commun. 2017. 8(1):1692.

17. Noguchi Y#, Yuan Z#, Bai L#, Schneider S, Zhao G, Stillman B*, Speck C*, Li H*. Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. Proc Natl Acad Sci U S A. 2017. 114(45):E9529-E9538.

18. Bai L#, Jastrab JB, Isasa M, Hu K, Yu H, Gygi SP, Darwin KH*, Li H*. Structural Analysis of Mycobacterium tuberculosis Homologues of the Eukaryotic Proteasome Assembly Chaperone 2 (PAC2). J Bacteriol. 2017. 199(9). pii: e00846-16.

19. Yuan Z#, Riera A#, Bai L#, Sun J, Nandi S, Spanos C, Chen ZA, Barbon M, Rappsilber J, Stillman B*, Speck C*, Li H*. Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 and Cdt1. Nat Struct Mol Biol. 2017. 24(3):316-324.

20. Bai L#, Yuan, Z., Sun, J., Georgescu, R., O’Donnell, ME., Li, H*. Architecture of the Saccharomyces cerevisiae Replisome. DNA Replication. Springer, 2017. 207-228. (book chapter)

21. Georgescu R#, Yuan Z#, Bai L#, de Luna Almeida Santos R, Sun J, Zhang D, Yurieva O, Li H*, O'Donnell ME*. Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation. Proc Natl Acad Sci U S A. 2017. 114(5):E697-E706.

22. Bai L#, Hu K#, Wang T#, Jastrab JB, Darwin KH*, Li H*. Structural analysis of the dodecameric proteasome activator PafE in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2016. 113(14):E1983-92.

23. Sun Q#, Han C, Liu L, Wang Y, Deng H, Bai L*, Jiang T*. Crystal structure and functional implication of the RUN domain of human NESCA. Protein Cell. 2012. 3(8):609-17.

24. Tang L#, Bai L#, Wang WH, Jiang T*. Crystal structure of the carnitine transporter and insights into the antiport mechanism. Nat Struct Mol Biol. 2010. 17(4):492-6.