柏林 博士

研究员，博士生导师

北京大学基础医学院生物化学与生物物理学系

地址：海淀区学院路38号北京大学基础医学院 邮编：100191

电话：010-82801414

E-mail: lbai@pku.edu.cn

实验室主要利用冷冻电镜等生物化学与生物物理学方法开展真菌药物靶点的结构与功能研究，以及基于结构的抗真菌药物研发。先后主持国家自然科学基金面上项目和国家海外高层次人才项目。近年来以第一/或通讯作者（含共同）在Nature，Nat Struct Mol Biol等国际一流杂志发表了多篇重要研究论文。

学习和工作经历

2002.9-2006.7 兰州大学生命科学学院 理学学士

2006.9-2012.8 中国科学院生物物理研究所 生物化学与分子生物学博士

2012.8-2016.7 美国布鲁克海文国家实验室 博士后

2016.7-2020.8 美国Van Andel研究所 高级研究科学家

2020.9至今 北京大学基础医学院 研究员

代表研究论文（通讯作者：*；第一作者：#）

1.Zhao C#, You Z#, Chen D#, Hang J#*, Wang Z, Wang L, Zhao P, Qiao J*, Yun C*, Bai L*. Structure of a fungal 1,3-β-glucan synthase. Sci Adv. 2023 Sep 15;9(37):eadh7820. doi: 10.1126/sciadv.adh7820.

2.Chen D#, Wang Z#, Wang L, Zhao P, Yun C*, Bai L*. Structure, catalysis, chitin transport, and selective inhibition of chitin synthase. Nat Commun. 2023 Aug 8;14(1):4776. doi: 10.1038/s41467-023-40479-4.

3.Zhao P#, Zhao C#, Chen D, Yun C, Li H*, Bai L*. Structure and activation mechanism of the hexameric plasma membrane H+-ATPase. Nat Commun. 2021 Nov 8;12(1):6439.

4.Bai L#*, Jain BK#, You Q#, Duan HD, Takar M, Graham TR*, Li H*. Structural basis of the P4B ATPase lipid flippase activity. Nat Commun. 2021 Oct 13;12(1):5963.

5.Bai L#*, You Q#, Jain BK#, Duan HD, Kovach A, Graham TR, Li H*. Transport mechanism of P4 ATPase phosphatidylcholine flippases. Elife. 2020 Dec 15;9:e62163.

6.Bai L*, You Q, Feng X, Kovach A, Li H*. Structure of the ER membrane complex, a transmembrane-domain insertase. Nature. 2020 Aug;584(7821):475-478.

7.Bai L*, Kovach A, You Q, Hsu HC, Zhao G, Li H*. Atomic structure of the eukaryotic lipid flippase Drs2p-Cdc50p. Nat Commun. 2019; 10:4142

8.Bai L#, Kovach A, You Q, Kenny A, Li H*. Structure of the eukaryotic protein O-mannosyltransferase Pmt1-Pmt2 complex. Nat Struct Mol Biol. 2019. 26:704-711

9.Bai L#, Wang T, Zhao G, Kovach A, Li H*. The atomic structure of a eukaryotic oligosaccharyl transferase complex. Nature. 2018; doi:10.1038/nature25755

10.Santos RLA#, Bai L#, Singh PK#, Murakami N#, Fan H, Zhan W, Zhu Y, Jiang X, Zhang K, Assker JP, Nathan CF, Li H*, Azzi J*, Lin G*. Structure of human immunoproteasome with a reversible and noncompetitive inhibitor that selectively inhibits activated lymphocytes. Nat Commun. 2017. 8(1):1692.

11.Noguchi Y#, Yuan Z#, Bai L#, Schneider S, Zhao G, Stillman B*, Speck C*, Li H*. Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. Proc Natl Acad Sci U S A. 2017. 114(45):E9529-E9538.

12.Bai L#, Jastrab JB, Isasa M, Hu K, Yu H, Gygi SP, Darwin KH*, Li H*. Structural Analysis of Mycobacterium tuberculosis Homologues of the Eukaryotic Proteasome Assembly Chaperone 2 (PAC2). J Bacteriol. 2017. 199(9). pii: e00846-16.

13.Yuan Z#, Riera A#, Bai L#, Sun J, Nandi S, Spanos C, Chen ZA, Barbon M, Rappsilber J, Stillman B*, Speck C*, Li H*. Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 and Cdt1. Nat Struct Mol Biol. 2017. 24(3):316-324.

14.Georgescu R#, Yuan Z#, Bai L#, de Luna Almeida Santos R, Sun J, Zhang D, Yurieva O, Li H*, O'Donnell ME*. Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation. Proc Natl Acad Sci U S A. 2017. 114(5):E697-E706.

15.Bai L#, Hu K#, Wang T#, Jastrab JB, Darwin KH*, Li H*. Structural analysis of the dodecameric proteasome activator PafE in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2016. 113(14):E1983-92.

16.Sun Q#, Han C, Liu L, Wang Y, Deng H, Bai L*, Jiang T*. Crystal structure and functional implication of the RUN domain of human NESCA. Protein Cell. 2012. 3(8):609-17.Tang L#, Bai L#, Wang WH, Jiang T*. Crystal structure of the carnitine transporter and insights into the antiport mechanism. Nat Struct Mol Biol. 2010. 17(4):492-6.

综述或书籍章节（通讯作者：*；第一作者：#）

1.Bai L*, Li H*. Structural insights into the membrane chaperones for multi-pass membrane protein biogenesis. Curr Opin Struct Biol. 2023 Apr;79:102563.

2.Bai L*, Li H*. Cryo-EM structures of the endoplasmic reticulum membrane complex. FEBS J. 2021 Feb 24. doi: 10.1111/febs.15786.

3.Bai L*, Li H*. Protein N-glycosylation and O-mannosylation are catalyzed by two evolutionarily related GT-C glycosyltransferases. Curr Opin Struct Biol. 2021 Jan 11;68:66-73.

4.Bai L*, Li H*. Cryo‐EM is uncovering the mechanism of eukaryotic protein N‐glycosylation. FEBS J. 2019; 286.9: 1638-1644.

5.Bai L#, Yuan, Z., Sun, J., Georgescu, R., O’Donnell, ME., Li, H*. Architecture of the Saccharomyces cerevisiae Replisome. DNA Replication. Springer, 2017. 207-228.